Package 'rWSBIM1322'

Title: Companion Package for WSBIM1322 Course
Description: Companion package for the WSBIM1322 course, distributing data and general documentation, and making course administration easier.
Authors: Laurent Gatto [aut, cre] , Julie Devis [ctb]
Maintainer: Laurent Gatto <[email protected]>
License: GPL-2
Version: 0.3.2
Built: 2024-10-30 05:36:07 UTC
Source: https://github.com/UCLouvain-CBIO/rWSBIM1322

Help Index


CPTAC data

Description

The data stems from the 6th study of the Clinical Proteomic Technology Assessment for Cancer (CPTAC). The authors spiked the Sigma Universal Protein Standard mixture 1 (UPS1) containing 48 different human proteins in a protein background of 60 ng/microL Saccharomyces cerevisiae strain BY4741. Two different spike-in concentrations were used: 6A (0.25 fmol UPS1 proteins/microL) and 6B (0.74 fmol UPS1 proteins/microL). In this subset, we limited ourselves to the data of LTQ-Orbitrap W at site 56. The data were searched with MaxQuant version 1.5.2.8, and detailed search settings were described in Goeminne et al. (2016). Three replicates peptide quantitation data are available for each concentration.

Usage

cptac_se

Format

An object of class SummarizedExperiment with 4051 rows and 6 columns.

Details

The data are available as SummarizedExperiment objects.

See the proteomics tutorial from the Bioinformatics Summer School 2019 (https://lgatto.github.io/bioc-ms-prot/bss-lab.html) for scripts on how these data were processed.

Examples

library("SummarizedExperiment")
data(cptac_se)
cptac_se
data(cptac_se_prot)
cptac_se_prot

Example for distances

Description

This is a small toy example providing expression values for 5 genes and three samples from Olga Vitek. It is used to compare euclidean and correlation distances and the effect/importance of scaling.

Usage

g3

Format

An object of class matrix (inherits from array) with 3 rows and 5 columns.

Examples

data(g3)
g3
matplot(t(g3), type = "b", xlab = "Samples", ylab = "Expression")

Expression data

Description

This data is a copy of the iris data, reframed for biomedical course. It illustrates the expression of 4 genes, BRCA1, BRCA2, TP53 and A1CF, in 150 patients, that have been categorised in 3 catégories, A, B and C.

Usage

giris

Format

An object of class data.frame with 150 rows and 5 columns.

Examples

head(giris)
pairs(giris, col = giris$GRADE)

pca2 <- prcomp(giris2[, -5], scale = TRUE)
factoextra::fviz_pca_ind(pca2, col.ind = giris2$GRADE)

Microarray expression data from Ohnishi et al. 2014.

Description

The is a subset of the full Hiiragi 2013 dataset from the Hiiragi2013 package. The data describes cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages.

Usage

data("hiiragi2013df1")
data("hiiragi2013df2")

Source

The data originally come from the Hiiragi2013 Bioconductor package. See inst/script/hiiragi2013.R to see how they have been converted.

References

Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages by Y. Ohnishi, W. Huber, A. Tsumura, M. Kang, P. Xenopoulos, K. Kurimoto, A. K. Oles, M. J. Arauzo-Bravo, M. Saitou, A.-K. Hadjantonakis and T. Hiiragi; Nature Cell Biology (2014) 16(1): 27-37. doi: 10.1038/ncb2881.

Examples

data(hiiragi2013df1)
data(hiiragi2013df2)

SummarizedExperiment data

Description

This is a small RNA-Seq data set, build from the data returned by the rWSBIM1207::kem2.tsv() function.

Usage

kem2_se

Format

An object of class SummarizedExperiment with 4774 rows and 16 columns.

Examples

library("SummarizedExperiment")
data(kem2_se)
assay(kem2_se)
colData(kem2_se)
rowData(kem2_se)

Generate data

Description

Generate data

Usage

make_data(noma)

Arguments

noma

'character(1)' that can be coerced into a numeric

Value

A SummarizedExperimet

Examples

make_data("123")

Metabolomics data (from MSMB)

Description

This data comes from the Modern Statistics for Modern Biology book and was originally called mat1 from the mat1xcms.RData file. It contains quantitation data for 399 metabolites and 6 knock-out and 6 wild-type samples.

Usage

metab1

Format

An object of class matrix (inherits from array) with 399 rows and 12 columns.

Examples

data(metab1)

dim(metab1)
metab1[1:10, 1:3]

Recap SummarizedExperiment data

Description

Several SummarizedExperiment datasets for the recap exercices in the conclusion chapter of the course.

Usage

recapSE1

Format

An object of class SummarizedExperiment with 1550 rows and 20 columns.

Examples

data(recapSE1)
recapSE1

data(recapSE2)
recapSE2

Package version

Description

Package version

Usage

rWSBIM1322version()

Examples

## check the package version that is currently installed
rWSBIM1322version()

Example data for t-tests illustration

Description

See scripts/randata.R for how these data were generated.

Usage

tdata1

Format

An object of class matrix (inherits from array) with 100 rows and 6 columns.

Examples

data(tdata1)
head(tdata1)

data(tdata2)
tdata2

data(tdata3)
tdata3

data(tdata4)
tdata4

Protein Uniprot identifiers

Description

A character vector with Uniprot identifiers.

Usage

data("up_selected")

Examples

data(up_selected)
head(up_selected)

Example data for PCA illustration

Description

See man/xy.R for how these data were generated. xy is scaled, xy0 is the orginal data.

Usage

xy

Format

An object of class data.frame with 20 rows and 2 columns.

Examples

data(xy)

xy0

xy
pca <- prcomp(xy)
summary(pca)
plot(pca)
biplot(pca)